EYEN
The expert in molecular imaging and nano-scale structural data analysis.
Our expertise lies in advanced computational analysis of proteins, biologics and nanoparticles for pharmaceutical research & development.
3D cryo-electron microscopy service
Celine 3D
3D cryo-electron microscopy service
- Comprehensive "Gene-to-Structure" workflow.
- Technique fuelled by unique advanced algorithms.
- Fast turnaround times.
- Ideal for protein complexes, biologics and nanoparticles.
service WORKFLOW
Celine 3D
Service workflow
Step 1: Submit sample
You prepare a sample that meets the following minimal analysis requirements and ship it to us:
- 100 uL sample volume at a concentration of 0.5 mg/mL
- Low ionic-strength buffer without cryo protectants and extensively polymerizing substances.
- Typical samples include drug-delivery nanoparticles (micelles, liposomes, oligo-based), viruses, membrane proteins, antibodies/epitopes and other biologics
Step 2: Sample screening
When the samples arrive in our imaging facility, we evaluate the behaviour of your sample under cryo conditions and:
- check for aggregation, optimal particle concentration, and complex stability,
- suggest Go/No-Go for the Analysis,
- give cost & time estimate of the Analysis.
Step 3: Submit order for analysis or resubmit sample
With green light from the sample screening, you submit an order for the analysis. We then:
- collect the 2D cryo TEM images on high-end electron microscope,
- estimate the final achievable resolution,
- provide you with initial trial model constructed from small subset of the collected data.
Step 4: Receive results - The 3D structural models
We perform the advanced iterative computational reconstruction and give you the 3D structural models of your complex that include:
- regular updates with intermediate results,
- robust resolution estimation,
- models of all identified conformations,
- fitting with X-ray models, if available.